Journal: PLOS One

Article Title: Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse model

doi: 10.1371/journal.pone.0346246

Figure Lengend Snippet: Number of ChAT + (A-C), GAD-67 + (D), Calbindin + (E), Parvalbumin + (F), Parvalbumin + −Calbindin + (G), Parvalbumin + −GAD-67 + (H), and ChAT + −GAD-67 + (I) cells per biological replicate. The number of cells analysed is the sum of immunostained positive cells from eight spinal cord cross-sections (two sections per lumbar level L3–L6) per biological replicate. Data are shown as mean ± standard deviation and were analysed by an ordinary one-way ANOVA. N = 3 biological replicates per genotype.

Article Snippet: Following three 10-min PBS washes at 100 rpm, secondary probing was performed for 3 hours in the dark in PBS containing 0.05% Tween-20 and the relevant secondary antibodies for ChAT (1:250 dilution, donkey anti-goat IgG H + L Alexa Fluor ® 405, Abcam, ab175664), Calbindin (1:500 dilution, Rhodamine TRITC-AffiniPure donkey anti-guinea pig Ig G H + L, Jackson Immuno Research, 706-025-148-JIR), Parvalbumin (1:500 dilution, Alexa Fluor 647-AffiniPure donkey anti-rabbit IgG H + L, Jackson Immuno Research, 711-605-152), and GAD-67 (1:500 dilution, donkey anti-chicken IgY H + L Highly Cross Adsorbed Alexa Fluor ® 488, Life Technologies, A78948).

Techniques: Standard Deviation